Home < Cnidium monnieri Research

Cnidium monnieri is a plant which grows in China (and possibly in Oregon) whose seeds are used in classical traditional Chinese medicine for skin problems and as a natural libido booster.
Cnidium seed contains several compounds including coumarins osthol or osthole, xanthotoxol, imperatorin, bergapten, monoterpene polyols, glucides and hepatoprotective sesquiterpenes.
Osthole has been found in studies to have anti tumor activity and to be helpful in strengthening bones.

Ready for a bedroom revolution?
Veyron-S will turn you into a stud!

We have customers who order Veyron-S for years and we are so sure about our product that we are ready to return back the money to you if you are not
satisfied with Veyron-S.
Money back guarantee!!!

Veyron-S is the best all-natural male enhancer available today.
This exciting new supplement improves sexual performance by:
Providing strong, long-lasting erections
Stimulating desire
Restoring natural vitality
Boosting stamina
The result: unforgettable orgasms.
Veyron-S is 100% free of the harmful synthetic chemicals found in other male enhancers. Users of this supplement do not suffer any deleterious side effects.
In clinical trials, the effects of Veyron-S lasted up to 60 hours, perfect for long weekends and romantic getaways.

Cnidium monnieri Research
Preparative isolation and purification of bergapten and imperatorin from the medicinal plant Cnidium monnieri using high-speed counter-current chromatography by stepwise increasing the flow-rate of the mobile phase
A high-speed counter-current chromatography (HSCCC) method was developed for the preparative separation and purification of bergapten and imperatorin from the Chinese medicinal plant Cnidium monnieri (L.) Cusson. The crude extract was obtained by extraction with ethanol from the dried fruits of Cnidium monnieri (L.) Cusson under sonication. Preparative HSCCC with a two-phase solvent system composed of n-hexane–ethyl acetate–ethanol–water (5:5:5:5, v/v/v/v) was successfully performed by increasing the flow-rate of the mobile phase stepwise from 1.0 to 2.0 ml min−1 after 180 min. The components purified and collected were analyzed by high-performance liquid chromatography. The method yielded 45.8 mg of bergapten at 96.5% purity and 118.3 mg of imperatorin at 98.2% purity from 500 mg of the crude extract in a single run. The recoveries of bergapten and imperatorin were 92.1 and 93.7%, respectively.
Keywords: Cnidium monnieri; Counter-current chromatography; Preparative chromatography; Pharmaceutical analysis; Plant materials; Bergapten; Imperatorin

Preparative isolation and purification of coumarins from Cnidium monnieri (L.) Cusson by high-speed counter-current chromatography
A high-speed counter-current chromatography (HSCCC) method for separation and purification of coumarins from Cnidium monnieri (L.) Cusson was developed by using stepwise elution with a pair of two-phase solvent system composed of light petroleum–ethyl acetate–methanol–water at volume ratios of 5:5:5:5, 5:5:6:4 and 5:5:6.5:3.5. Five kinds of coumarins were obtained and yielded 7.6 mg of xanthotoxin(I), 7.6 mg of isopimpinellin(II), 9.7 mg of bergapten(III), 60.5 mg of imperatorin(IV), 50.6 mg of osthole(V) and 10.2 mg of one unknown compound from 150 mg crude sample. The purity of these compounds was 95.0%, 99.6%, 99.7%, 100%, 100% and 98.1%, respectively, as determined by HPLC. Their structures were identified by 1H NMR.
Keywords: Counter-current chromatography; Preparative chromatography; Cnidium monnieri (L.) Cusson; Osthole; Imperatorin; Bergapten; Xanthotoxin; Isopimpinellin

Analysis of Cnidium monnieri fruits in different regions of China
Application of multivariate data analysis has become a popular method in the last decades, mainly because it can provide information not otherwise accessible. The information includes classification, searching similarities, finding relationships, finding physical significance to principal components, etc. Fifty three Chinese medicinal herbs of eleven kinds of coumarins were collected and determined by HPLC. The results were studied by principal component analysis (PCA) and cluster analysis. It was showed that the samples could be clustered reasonably into different groups, which correspond with variation of regularity and distribution of coumarin of Cnidium monnieri fruits in different regions of China.
Author Keywords: Cnidium monnieri; Coumarin; PCA; Cluster analysis

Identification and structure-activity relationship studies of osthol, a cytotoxic principle from Cnidium monnieri
Osthol (1) was isolated from the fruit of Cnidium monnieri as a cytotoxic principle. Its structure-activity relationship study reveals that the 3,4-olefinic bond is essential for its cytotoxic activity, and the prenyl (C5) unit attached at the 8 position enhances the cytotoxicity. Analogues 7 and 8 that have a longer alkoxy unit at the 7 position showed ten times higher cytotoxicity than 1.

Chromones from Cnidium monnieri
Four new chromones, cnidimol C–F, along with cnidimol A, cnidimol B and karenin were isolated from the aerial parts of Cnidium monnieri. The structures of the new chromones were determined to be 5,7-dihydroxy-2-hydroxy-methylchromone, 5,7-dihydroxy-6-[(2Z)-3-hydroxymethyl-2-butenyl]-2-hydroxymethylchromone, 5,7-dihydroxy-6-[2-hydroxy-3-methyl-3-butenyl]- 2-hydroxymethylchromone and 5,8-dihydroxy-2-hydroxymethyl-8-methyl-4H,9H-pyrano [3,2-h][1]benzoxepin-4-one.
Author Keywords: Cnidium monnieri; Umbelliferae; chromone; cnidimol A–F; karenin

Fingerprint analysis of the fruits of Cnidium monnieri extract by high-performance liquid chromatography–diode array detection–electrospray ionization tandem mass spectrometry
A method incorporating high-performance liquid chromatography (HPLC) with electrospray ionization (ESI) and tandem mass spectrometry (MS), with parallel analysis by HPLC with UV detection using a diode-array detector (DAD) was developed for the qualitative characterization of coumarin and chromone constituents in the fruits of Cnidium monnieri. The chromatographic separations were performed on a Diamonsil™ C18 column (4.6 mm × 200 mm, 5 μm) with water with 50 mM ammonium acetate and 2% acetic acid (A) and acetonitrile (B) as the mobile phase. According to the characteristic UV spectra, the information of molecular weight and structure provided by ESI–MS/MS, 13 coumarin and 7 chromone components were detected and identified. This method is rapid and reliable for identification of the constituents in the complex herbal system, and the fragmentation patterns proposed could be extended to the similar compounds.
Keywords: HPLC–DAD–ESI–MS/MS; Coumarin; The fruits of Cnidium monnieri; Fingerprint analysis

Petroleum ether extract of Cnidium monnieri ameliorated scopolamine-induced amnesia through adrenal gland-mediated mechanism in male rats
Aim
Our previous study indicated petroleum ether layer of Cnidium monnieri L. Cuss. (CM) and its ingredient osthole could alleviate scopolamine-induced amnesia in female rats.
Materials and methods
Hence, this study was desired to investigate the mechanism of the ameliorating effects of petroleum ether layer of CM on the performance impairment of inhibitory avoidance task and Morris water maze induced by scopolamine in male rats.
Results
Cnidium monnieri L. Cuss. at 0.1–0.6 g/kg orally administered 60 min before the training trial ameliorated the scopolamine-induced performance impairment on inhibitory avoidance learning and water maze in male rats. Only adrenalectomy but not peripheral cholinergic antagonist scopolamine methylbromide and catecholaminergic neurotoxin 6-hydroxydopamine blocked the ameliorating effects of Cnidium monnieri L. Cuss. on scopolamine-induced performance impairment in rats.
Conclusion
Therefore, we demonstrated that the ameliorating effects of Cnidium monnieri L. Cuss. on scopolamine-induced performance impairment may be related to activating the adrenal gland and central acetylcholingeric neuron, instead of peripheral nervous system.
Keywords: Cnidium monnieri L. Cuss.; Scopolamine; Inhibitory avoidance task; Morris water maze; Adrenalectomy

Micelle-mediated extraction and cloud-point preconcentration of osthole and imperatorin from Cnidium monnieri with analysis by high performance liquid chromatography
A new method based on micelle-mediated extraction and cloud-point preconcentration was developed for the separation and determination of hydrophobic compounds osthole and imperatorin from Cnidium monnieri by high performance liquid chromatography with photodiode array detection. The non-ionic surfactant C13E8 (Genapol X-080) was chosen as the extract solvent.

We bring you the highest quality original product from Hong Kong.
Achieve more satisfying sexual experiences with Veyron-S !!!
Order Veyron-S now for only USD29.95